Suppress Nematodes to Improve Leaf Photosynthesis, Yield, and Grape Quality

Suppress Nematodes to Improve Leaf Photosynthesis, Yield, and Grape Quality

S. Kaan Kurtural, PhD, Senior Agronomy Information Technology Manager

  • Employ® supports nematode control in vineyards and helps reduce the effects of nematode feeding
  • Improved leaf photosynthesis was linked to stronger yield, higher Brix, and better grape quality in multi-site trials
  • As one of today’s agricultural biologicals, Employ helps support plant health, water status, and berry composition

Employ® is a biochemical pesticide that is foliarly applied and can help support nematode control in vineyards by helping plants overcome the effects of nematode feeding. In multi-year, multi-site trials, Employ was shown to improve leaf photosynthesis, plant water status, yield, sugar accumulation in berries, as well as plant secondary metabolites such as anthocyanins in black-skinned wine grapes.

Employ is a second-generation αβ Harpin protein and fits within a broader category of agricultural biologicals. These proteins are produced by many pathogenic bacteria that can signal the plant’s immune system to upregulate its defense pathways. This resulted in improved leaf photosynthesis therefore improved growth and amelioration of berry composition including greater amounts of phenolic biosynthesis.

In replicated independent trials (176PS23VINCA1), the results indicated a reduction of nematode populations in the rhizosphere (Figure 1), increased leaf photosynthesis throughout the season (Figure 2), greater yield (Figure 3), higher Brix (Figure 4), and higher anthocyanins in berry skin (Figure 5) in wine grapes grown on susceptible Coudrec 3309 rootstock in California at two different sites.

Field Trials Show Improved Leaf Photosynthesis, Yield and Brix

Figure 1: Root-knot and ring nematode counts in grapevine rhizosphere before and after Employ application
Figure 1. Root knot and ring nematode counts in grapevine rhizosphere before and after Employ application 2023-2024
in Solano and Santa Barbara Counties in California (p<0.0001).
176PS23VINCA1

Figure 2: Improved leaf photosynthesis in grapevines after Employ application across California trial sites
Figure 2. Improvement of leaf photosynthesis over the growing season 2023-2024 with Employ application in Solano and Santa Barbara Counties
in California (p<0.0001)
. 176PS23VINCA1

The suppression of nematode feeding on roots of grapevines led to:

  • 35% greater leaf photosynthesis
  • 30% greater yield per acre
Figure 3: Higher grapevine yield following Employ application in vineyard field trials
Figure 3.  Effect of Employ on yield of grapevine 2023-2024 in Solano and Santa Barbara Counties in California (p<0.0001) 176PS23VINCA1

Nematode Control Supports Sugar Accumulation and Grape Quality

Greater leaf photosynthesis is associated with higher sugar export from grape leaves during ripening. Likewise, improved nematode control and higher photosynthate export were associated with greater sugar loading in grape berries.

Figure 4: Increased grape berry Brix after Employ application in multi-site vineyard trials
Figure 4.  Effect of Employ on grape berry Brix in 2023-2024 in Solano and Santa Barbara Counties in California (p<0.0001). 176PS23VINCA1

There is a direct linear relationship between sugar accumulation in the grape berry and anthocyanin (color) formation in the skin.  Likewise, due to nematode control and better sugar loading into the grape berry, higher skin anthocyanin was achieved in Cabernet Sauvignon and Pinot Noir berries (Figure 5).

Figure 5: Higher grape berry skin anthocyanin following Employ application in wine grape trials
Figure 5.  Effect of Employ on grape berry skin anthocyanin (mg/berry) 2023-2024 in Solano and Santa Barbara Counties
in California (p<0.0001).
176PS23VINCA1

Best Use Recommendations for Employ in Vineyards

For growers looking to improve nematode control with agricultural biologicals, Employ can be incorporated at key timings throughout the season.

TimingRate
Post-Harvest2 oz/Acre
3-5 mm Berry2 oz/Acre
Bunch Closure2 oz/Acre
Verasion2 oz/Acre

Field comparison of Untreated Control and Employ applied at 2 oz/Acre.

Berry comparison untreated: Cabernet Sauvignon berries at harvest without Employ treatment
Cabernet Sauvignon grapevine berries
at harvest without Employ.
Berry comparison treated: Cabernet Sauvignon berries at harvest with Employ applied at 2 ounces per acre
Cabernet Sauvignon grapevine berries
at harvest with Employ 2 oz/Acre.

Originally published: May 4, 2025